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Description

Alcohol is associated with increased mortality and morbidity. Pulmonary infections with opportunistic pathogens can occur in healthy humans; however, hazardous alcohol exposure like binge alcohol intoxication (≥ 0.08% BAC) is a major risk factor. We’ve previously investigated the efficacy of Sulforaphane (SFN) prophylaxis in protecting human and murine alveolar macrophage function against opportunistic infections when exposed to binge alcohol conditions in vitro. However, an in vivo model investigating SFN and hazardous alcohol exposure in the context of infection is not well understood. The aim of this study is to 1) establish a G. mellonella larvae model by determining the LD50 of alcohol, SFN, Gram-Positive S. epidermidis, and Gram-Negative B. thailandensis and 2) test the protective effects of SFN against a bacterial infection after hazardous alcohol exposure.

Disciplines

Allergy and Immunology | Biological Phenomena, Cell Phenomena, and Immunity | Immune System Diseases | Inorganic Chemicals | Medical Toxicology

Keywords

Sulforaphane, Innate Immune System, Galleria mellonella, alcohol

Document Type

Poster

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A Galleria mellonella Study: Protective Effects of Sulforaphane on Immune Function in Binge Alcohol Exposure

Alcohol is associated with increased mortality and morbidity. Pulmonary infections with opportunistic pathogens can occur in healthy humans; however, hazardous alcohol exposure like binge alcohol intoxication (≥ 0.08% BAC) is a major risk factor. We’ve previously investigated the efficacy of Sulforaphane (SFN) prophylaxis in protecting human and murine alveolar macrophage function against opportunistic infections when exposed to binge alcohol conditions in vitro. However, an in vivo model investigating SFN and hazardous alcohol exposure in the context of infection is not well understood. The aim of this study is to 1) establish a G. mellonella larvae model by determining the LD50 of alcohol, SFN, Gram-Positive S. epidermidis, and Gram-Negative B. thailandensis and 2) test the protective effects of SFN against a bacterial infection after hazardous alcohol exposure.