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B26: Fastidious Bacteria Grow Better in the Right Media
Fastidious Bacteria Grow Better in the Right Media
Purpose: Nisseria gonorrheae is a common bacteria that causes a sexually transmitted disease. This disease can cause significant discomfort for both men and women, which can lead to infertility. Treatment of the bacterium has been a simple matter of diagnosis and antibiotic treatment in the past, but now this bacterium is becoming increasingly antibiotic resistant because of excessive antimicrobial treatments. A novel way to treat N. gonorrheae infection involves focusing on finding bacteriophage that targets N. gonorrheae. N. gonorrheae broth cultures are used to enrich for bacteriophage; but the current standard medium for growing N. gonorrheae, Fastidious Broth, was not allowing the bacteria to grow within the optimal range of 8-12 hours.
Methods: The difficulties with growing N. gonorrhea samples in Fastidious Broth led us to search for other media options that would result in bacteria growth in a more timely manner. This experiment characterized different growth media supplementation options with the aim of developing a medium that allows for completion of a bacterial growth curve over the course of 8-12 hours, which would be best suited for bacteriophage isolation and amplification protocols. To do this we grew colonies of fastidious bacteria including N. gonorrheae, S. pneumoniae, and F. novacida in liquid media, taking OD readings hourly during the bacterial growth. The media used was Brain Heart Infusion (BHI) using three different modifications of BHI (A, B, C).
Results: N. gonorrheae, S. pneumoniae, and F. novacida showed consistent growth rates in the liquid media, with Fastidius Broth as the control. N. gonorrheae showed the best growth in BHI supplementation medium C.
Conclusions: Our findings demonstrate that the supplement medium we developed, BHI supplementation media C, is the most optimal for N. gonorrheae to grow from lag phase to stationary phase in the shortest amount of time. That makes this medium better for use in laboratory experimentation.
Keywords: Nisseria gonorrheae, antibiotic resistance, bacteriophage, bacterial growth curve, Fastidious Broth, log phase, amplification